When 5-15 pieces of stem have fallen onto the plate, the sterile tweezers can be used to manipulate and move the pieces of Cannabis stem. They should be placed equidistant from one another and gently pushed down to ensure complete contact with the media. The lid can then be placed back onto the Petri dish. Parafilm, a stretchy plastic film, is wrapped along the edge of the plate and its lid. This helps in retaining moisture and keeping the contents sterile.
c-ray;92626 said:breeding is a subjective thing, it would be easy if thc was the only thing we are interested in, but it is not...so that is why it is important to grow the plants more than once, and ideally all side by side, and even in different gardens by different gardeners... and then tested (bioassayed) by as many folks as possiblethe biochemical ratios will different in every scenario, I guarantee it, they will even change in time as the plant is grown a few times from cloneseeds are highly variable, there is really no need for everything to be homogenous only that it is good
Why does GW cultivate its own cannabis plants?A.The absolute requirement for a plant-based medicine from a regulatory point of view is "control of starting materials". A drug in its manufacture goes through many processes, each of which need to be monitored and strict quality controls applied. This process control and QC would be invalidated if the starting materials were of poor or inconsistent quality. Laboratory analysis of GW's selected chemotype lines demonstrates that the cannabinoid ratios are very consistent. Such high levels of consistency are unusual in plants and are very important in applications made to medical regulatory authorities.GW's foremost consideration therefore is the cultivation of highly consistent plants with defined cannabinoid ratios. We have a number of chemotypes (varieties characterised by their chemical content) chosen for their composition and morphological traits.GW cultivates cannabis in order to be able to control the starting materials for the in-house production of GW's cannabinoid pharmaceutical products. GW does not distribute herbal cannabis to outside researchers or institutions.